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A new insulator element to preserve expression of randomly integrated transgenes
Expression of randomly integrated transgenes depends on DNA structure adjacent to the integration site. We have characterized a mammalian DNA sequence of only 65 base pairs able to preserve expression of the transgene independently of its local environment. Such sequences are called insulator elements.

Technical Description :
Compacted chromatin also called heterochromatin limits gene transcription. Our insulator element isolates transgene integration site from local negative influence of chromatin structure. Moreover it allows to limit the regulatory effect of transcription activating or repressing sequences cis-located. It blokes interaction between the regulatory element and the promoter of the transgene when it is located between both elements. This protection effect is independent of the orientation of the insulator DNA sequence which does not regulate negatively or positively transgene expression.

Potential applications :
- Production of recombinant proteins - Production of trangenic animals - Gene transfert - Gene therapy

Benefits :
We have identified an insulator element which is a DNA fragment of only 65 base pairs long. It protects efficiently transgene expression against cis-regulatory elements and negative influence of chromatin structure (heterochromatine, telomere region).

Intellectual property :
Patent pending in PCT (FR0756890) Priority date : August 2, 2007

Partnership type :
Licensing