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		<title>Technology Offers  - OSEO</title>
		<description>The 10 newest offers</description>		<link>http://www.technologie.oseo.fr</link>		<item>
			<title><![CDATA[Characterization of a new Competence-Stimulating Peptide and growth conditions allowing natural transformation of Streptococcus thermophilus strains used in dairy industry]]></title>
			<description><![CDATA[INRA’s research team “Peptides et communication bactérienne” (MICALIS unit) steered by Dr. Véronique Monnet has identified a method for obtaining improved Streptococcus thermophilus strains by a natural process of horizontal gene transfer. This method would be of particular interest for the dairy industry which is always looking for new S. thermophilus strains for the yoghurt and cheese production. The world-wide dairy market makes these S. thermophilus starter strains of a major economic importance and improvement of their properties is continuously sought by dairy manufacturers.

First, INRA’s researchers have found growth conditions triggering bacterial competence. They have demonstrated that the natural transformation of a model S. thermophilus strain can be turned on spontaneously in a peptide-free chemically defined medium during a narrow window of the exponential growth phase - the growth medium composition influencing the competence state of Streptococcus species. 
Then, using a proteomic approach, INRA’s researchers have identified and characterized a competence-stimulating peptide, a new peptide implicated in the control of the competence of S. thermophilus. Interestingly, the use of a synthetic competence-stimulating peptide or overexpression of the gene encoding it increases the transformation rate of poorly competent S. thermophilus strains and made it possible to transform non competent S. thermophilus strains. 

Based on these findings, protocols of induction of competence and natural transformation of S. thermophilus strains comprising the chemically defined medium, the competence-stimulating peptide, and a donor DNA of interest can be implemented by the dairy manufacturers for improving starter strains with industrially relevant properties such as acidification rate, texture production, phage resistance, etc.

Available

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			<pubDate><![CDATA[Fri, 11 May 2012 17:38:04 +0200]]></pubDate>
			<link>http://www.technologie.oseo.fr/repertoires_d_offres/6/6_2/6_2_3/ot_it_sma12?lang=ang</link>
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			<title><![CDATA[On-line monitoring of the fluence of modulated photon beams upstream to the patient in radiotherapy: a transparent detector design]]></title>
			<description><![CDATA[The invention relates to an original design which allows a full coverage of the monitored area with no dead zone. No inhomogeneity is introduced in the beam being measured upstream to the patient.

The principle of the device is based on air vented cells, in a checkered pattern. Each independent sensitive cell operates as an ionisation chamber. A tiny portion of the incident photons interact in a thin converter, generating fast primary electrons. Those electrons ionise the sensitive volume and the ions drift towards the electrodes. The charge collected on the electrodes is related to the beam fluence, being measured in 2D. As primary electrons are emitted with an angle with respect to the incident photon, ionisation can occur far from the photon interaction point, washing out the spatial information. The pillars of the checkered pattern will stop the
electrons emitted with a large angle, preserving the spatial information. The low density pillars have the same area as the sensitive cells. As the checkered pattern is present on two complementary floors, the whole surface is monitored with no dead zone and the beam is attenuated uniformly. 
BENEFITS
The two floors checkered pattern allows a complete coverage of the area, does not introduce inhomogeneity in the beam to be measured and minimizes the beam attenuation. If high gradient zones are present in the beam, they are monitored regardless of their location. In addition, the double floor measurement can be used to point out a wrong setting of the beam energy.
Using such a transparent detector to monitor and record the incoming photon beam during radiotherapy sessions, the patient safety can be increased. The device is sensitive enough to monitor the beam at the linac pulse scale, in real time. The beam delivery could be stopped if a mismatch with respect to the planned treatment is being noticed.


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			<pubDate><![CDATA[Sat, 28 Apr 2012 00:17:49 +0200]]></pubDate>
			<link>http://www.technologie.oseo.fr/repertoires_d_offres/6/6_1/6_1_12/04084_01?lang=ang</link>
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			<title><![CDATA[Memristif element and electronic memory based on such element]]></title>
			<description><![CDATA[Recent work done on type materials LixCoO2 helped highlight a resistive reversible switching of a sandwich consisting of a degenerate semiconductor electrode, a thin layer of material and metallic counter electrode. 
The reversibility of the red/ox process can be considered very reliable and speeds fast switching. 
The material is commonly used in the battery industry and therefore easily integrated into a conventional
manufacturing process. The analysis shows that the nature of the electrodes used does not affect the physical
processes involved.
Ongoing experiments will complete the technical specification of the structure.
BENEFITS
Using this type of structure whose switching is very fast, reversible and the method of manufacture now well controlled, allow the production of &nbsp;&nbsp;high-density non-volatile memories integrable in the manufacture of microelectronic components.

The main application field of this type of compound is in the manufacture of integrated circuits and more particularly in the field of rapid and non-volatile memories, and also in complex circuits using memories buffers
(FPGAs, microprocessors...).


]]></description>
			<pubDate><![CDATA[Fri, 27 Apr 2012 00:18:25 +0200]]></pubDate>
			<link>http://www.technologie.oseo.fr/repertoires_d_offres/1/1_1/1_1_4/01323_02?lang=ang</link>
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			<title><![CDATA[Electro-colloidal lithography: A versatile approach combining colloidal particles and electric fields for the fabrication of patterned polymer and metal films]]></title>
			<description><![CDATA[CONTEXT
Colloidal lithography, which is based on the use of two-dimensional colloidal crystals as masks or templates, was developed recently to offer an alternative to more conventional lithography techniques such as&nbsp; photolithography, electron beam lithography, dip pen lithography, and nano-imprinting that usually suffer from low throughput and high cost.
However, the production of patterned surfaces by colloidal lithography still requires traditional nanofabrication processes such as evaporation, etching, or even photolithographic processes which make actual colloidal lithography not so advantageous. Moreover, when non-close-packed colloidal crystals are desired for patterning, additional steps such as e.g. particle etching, substrate dilatation, production of a template to direct the assembling or transfer printing are carried out. These additional steps make the industrialization of the process difficult. We developed a new process that is straightforward, easy and cost-effective to produce patterned polymer and metal surfaces.

&nbsp;The technique named electro-colloidal lithography requires only an AC/DC field generator and colloidal&nbsp;particles. At first colloidal particles are inserted into an electro-chemical cell and organized into lattice of different geometries (hexagonal, chains...) on an electrode controlling the distance between particles. Once the desired distance is obtained, the organized particles can be stuck on the substrate forming the colloidal mask. Then a second population of particles is inserted into the electro-chemical cell and a DC electric field
is applied. Depending on the nature and concentration of the colloidal particles inserted, patterned surfaces of different geometries - spheres, hollow spheres, rings or holes- and compositions polymer or metal- are
obtained. Any types of electrode can be used. 
BENEFITS
The technique is versatile, easier, more cost-effective and more straightforward than conventional lithography techniques. The fact that no additional treatments are required, to obtain a large variety of patterned substrates, reduces the complexity and fabrication costs. This process is environmentally friendly since no organic solvent are required. The nature of the substrate can be changed to fit the application requirements as long as it is conductive. The colloidal particles used to pattern the surface as well as the electrodes being commercially available, the scale-up of this process can be conceivable. When micrometric or sub-micrometric scales are needed, this technique can offer a very interesting alternative to standard lithography techniques.
This technique could be employed for optoelectronic devices, analytical chemistry, surface treatment, chemical sensors and biotechnology.


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			<pubDate><![CDATA[Fri, 27 Apr 2012 00:18:23 +0200]]></pubDate>
			<link>http://www.technologie.oseo.fr/repertoires_d_offres/2/2_2/2_2_14/04129_01?lang=ang</link>
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			<title><![CDATA[PROCESS FOR TRANS-RESVERATROL SYNTHESIS]]></title>
			<description><![CDATA[CONTEXT
The presence of trans-resveratrol (3,4,5-trihydroxystilbene) in about sixty plants and therefore in our daily diet is well-known. This polyphenol offers a broad range of biological activities especially cardioprotective, neuroprotective and cancer chimioprotective effects. Owing to its antioxidant activity, the trans-resveratrol is now currently used in anti-aging cream and food additive. The trans-resveratrol can be extracted from asiatic plants but this process requires large amounts of plants and solvents. The prior art describes several methods of synthesis of trans-resveratrol and derivatives including Heck coupling and Wittig reaction. Heck reaction is carried out in presence of Pd catalyst which is inconvenient for synthesis of cosmetics or drugs. The main problem of Wittig reaction applied to the synthesis of polyphenols is the protection and deprotection reactions of phenolic functions of starting aromatic compounds towards basic medium. The reactants of protection and deprotection currently used in prior art are expensive and harmful and some deprotection reactions afford decomposition compounds (see in table below).





Protective groups


Reactants for

deprotection reactions


Comments



MOM =

-O-CH2-O-CH3


AcOH/H2SO4

NaI / Me3SiCl


Reactant of protection reaction MOM-Cl is
  carcinogenic



-CH3


BBr3

Py, HCl


BBr3&nbsp; is a toxic, inflammable and
  explosive reactant.



-CH2Ph


Hydrogénation

PhNMe2


Reactant of protection reaction PhCH2Br
  is lachrymatory



-SiMe3


H2O,
  EtOH


The protective group is sensitive towards protic
  solvents and bromation réactants.



-SiMe2tBu


nBu4NF

HF&nbsp;/ NaF


Deprotection reaction with nBu4NF can
  afford decomposition products. The protective group is sensitive towards
  bromation réactants.


This invention describes a new process for trans-resveratrol synthesis from 4-hydroxybenzaldehyde and 3,5-dihydroxybenzylic alcohol by using a cheap and non toxic reactant for the protection reaction which does not react with benzylic alcohol function. The protective groupe is proving insensitive towards one hand bromation reactant used for the transformation of benzylic alcohol into benzylic bromide and the other hand, the base used in Wittig reaction. An additional deprotection step is not necessary, this reaction occurs during the&nbsp;aqueous work-up of the reaction.
BENEFITS
This new process for the synthesis of trans-resveratrol can be applied to synthesis of other polyhydroxylated stilbenes which present biologicalactivities. The reactants required for the protection and deprotection of
phenolic functions are cheap, no toxic and no polluting. A specific reaction of deprotection is not necessary. The intermediate compounds are purified by crystallysation. This new general, eco-friendly and economical
process for synthesis, of polyhydroxylated stilbenes can be easily implemented on industrial scale.
This process has been developed for the synthesis of a few grams of trans-resveratrol and can be easily scaled up or adapted to other polyhydroxylated stilbenes.
This process can prompt in the field of biology an industrial production of the trans-resveratrol and its derivatives which are promising molecules due to their numerous therapeutic and anti-fungal effects.


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			<pubDate><![CDATA[Wed, 25 Apr 2012 00:18:23 +0200]]></pubDate>
			<link>http://www.technologie.oseo.fr/repertoires_d_offres/5/5_2/5_2_4/04383_01?lang=ang</link>
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			<title><![CDATA[A NOVEL PLASMID for the INSERTION of a SEQUENCE in a BACTERIAL CHROMOSOME BY HOMOLOGOUS RECOMBINATION]]></title>
			<description><![CDATA[CONTEXT
1)&nbsp;&nbsp;&nbsp;&nbsp; Methodology to insert a DNA fragment in a bacterial chromosome
The most widely used method consists in transforming a bacteria with a plasmid containing the genetic sequence of interest, an antibiotic resistance gene, and the sac B gene, coding a toxic enzyme in E coli.,. Transformed bacteria containing the plasmid integrated by homologous recombination are selected on a medium containing the antibiotic at a non permissive temperature of 44°C to select&nbsp; bacteria. Then bacteria are screened to detect plasmid excision events thanks to the sacB gene expression. 
This method doesnt systematicaly work well and is not convenient when one works with thermosensitive bacteria, which have a low growth rate between 42-44°C. Moreover these systems dont allow to differentiate mis-replicative events of episomal plasmids from events of plasmid integration. And the frequency of plasmid integration is in the same range than the frequency of sponteneous antibiotic resistance coming from chromosomal mutations.
2)&nbsp;&nbsp;&nbsp; The Host Killing Protein
&nbsp;(HOK) is known to be highly toxic to membrane pores of bacterial cells. It is used in Harms system as a marker of a successful homologous recombination event.
&nbsp;

Inventors have demonstrated that HOK gene, when coming from a single copy doesnt disturb the bacterial growth, whereas it becomes toxic when coming from several copies. This discovery allows to differentiate events of
autonomous replication of the plasmid from events of integration of the plasmid into the chromosomal target by homologous recombination.
They have developped a system allowing :
-&nbsp; To replace a gene in a bacterial chromosome in a targeted site of the chromosome
-&nbsp; to induce homologous recombination by several orders of magnitude by the presence of various DNA lesions on the novel plasmid.
-&nbsp; To work with thermosensitive bacteria at high temperatures
BENEFITS
-&nbsp; easy to settle and improves homologous recombination and cell selection of final transformed bacteria 
-&nbsp; Induction of the excision of the plasmid in liquid medium 
-&nbsp; Possibility to work with E coli, Pseudomonas putida&nbsp;and probably other gram- bacteria.
This invention is useful in the field of research tools. The main applications of the system concern the molecular biology and genetic engineering, to study and integrate genetic sequences in a bacterial chromosome.
DEVELOPMENT STAGE
The system has been validated in E coli. Mutations have been generated by deletion (250 bp) insertion (1,2kb), substitutions (1 or 2 bp). These mutations have been checked by PCR  sequencing.


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			<pubDate><![CDATA[Sat, 07 Apr 2012 00:18:19 +0200]]></pubDate>
			<link>http://www.technologie.oseo.fr/repertoires_d_offres/6/04012_01?lang=ang</link>
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			<title><![CDATA[BIO-BASED POROUS ORGANOGEL AS FLUID ADSORBANT MATERIAL]]></title>
			<description><![CDATA[CONTEXT
Currently used adsorbant materials present many disadvantages:
-They are known to get quickly saturated on their surface not allowing then the trapping of fluids in high quantity;
-Their density, their porosity as well as their polarity cannot be controlled;
-Currently available adsorbant porous materials are either mineral-based (activated carbon, zeolite, clay) or synthetic polymers;
-They are also hard to recycle which leads to additional costs;
-Finally trapped products are known to be hard to extract from said materials.
&nbsp;In the present work, the inventors have developped the use of microporous bio-based organogels as fluids
(air, water containing organic compounds) adsorption-based trapping materials and / or as fluids controlled liberating materials after solubilization of said fluids in the organogels.
Organogels are semi-solid materials comprising an organic liquid or an oil immobilized in a tridimensional network of fibers resulting from the self-assembly of an organogelling agent either polymeric or of a low moleculary mass. As a consequence of the targetted products to be trapped, the developped porous organogels are hydrophobe and comprises an oil (soybean oil)
DEVELOPMENT STAGE
The use of said organogels has been assessed in numerous applications:
-Trapping of an hydrocarbon film on the surface of water;
-Trapping of polluting agents on a soil;
-Trapping of an odor;
-Controlled release of an entrapped fluid;

BENEFITS
The technology offers many advantages versus current technologies:
-Bio-based products;
-High specific surface area due to &nbsp;which leads to better adsorption property;
-Controlled density, porosity and polarity.
-Dual property : adsorption / solubilization à higher fluid trapping load;
-Inexpensive and eco-friendly process;
Two types of organic compounds contained in fluids are targetted&nbsp;:
-Polluting agents (hydrocarbon, xenobiotics, heavy metal ions)
-Volatil substances (insect pheromones, plant, flower or resin aroma)
The trapped fluid can then be directly analysed through liquid or gaz chromatography and / or MS after
dissolution in a appropriate solvent. The porous aerogel can also play the role of a SPE support.


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			<pubDate><![CDATA[Fri, 06 Apr 2012 00:18:26 +0200]]></pubDate>
			<link>http://www.technologie.oseo.fr/repertoires_d_offres/10/10_2/10_2_1/04856_01?lang=ang</link>
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			<title><![CDATA[HIGHLY SENSITIVE PRION BLOOD DETECTION TEST]]></title>
			<description><![CDATA[CONTEXT
Transmissible spongiform encephalopathies (TSE - Creutzfeldt-Jakob disease in humans, BSE, scrapie, CWD in animals) are characterized, post-mortem, by accumulation of the pathological prion protein PrPSc in brain tissue. This unconventional infectious agent is transmitted by blood transfusion, contaminated feed, food or health products. 
So far, no commercially blood screening assay is available to detect prion agent in blood. Indeed, standard procedures used to detect PrPsc in tissues have failed in blood samples. Several studies have suggested that
the infectious forms circulating in blood may have different physicochemical properties from those accumulating in tissues, especially concerning their high sensitivity to proteinase K digestion.

The present invention relates to the use of a family of thienyl pyrimidine compounds that induce a specific oligomerization of PrPSc dimers and trimers through aggregation/stabilization, allowing efficient amplification of blood PrPSc for its reliable detection in various samples and early diagnosis of TSE. 
BENEFITS
Our technology provides: 
- a very high sensitivity and specificity of PrPSc detection compared to standard procedures (x1000000), which may apply to various detection technology (western-blotting, Elisa, fluorescence technology)
- a potential ante-mortem diagnosis from biological fluids and poorly concentrated tissues with rapid amplification of PrPsc without the need of proteinase K digestion step.
The mains applications of this technology are : 
- blood tests for Creutzfeldt-Jakob diseases and possibly other human prionopathies (Parkinsons, Alzheimers and Huntingtons diseases)

- blood tests for animal prion diseases, post-mortem and ante-mortem tests on tissues for veterinary purposes
- safety of blood products (93 millions blood donations annually)
DEVELOPMENT STAGE
This technology has already proved its efficiency on blood samples spiked with contaminated brain&nbsp; homogenates. Studies are ongoing on blood samples from a prion hamster model and from human patients.
A manufacturable kit is in preparation to allow easy, fast and cheap screening of blood products.
PUBLICATION
Oligomeric-induced activity by thienyl pyrimidine compounds traps prion infectivity. Ayrolles-Torro A et. al. J Neurosci. 2011 Oct 19;31(42):14882-92.


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			<pubDate><![CDATA[Fri, 06 Apr 2012 00:18:24 +0200]]></pubDate>
			<link>http://www.technologie.oseo.fr/repertoires_d_offres/6/6_1/6_1_6/00425_01?lang=ang</link>
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			<title><![CDATA[WASTE WATER DECONTAMINATION PROCESS WITH POLYELECTROLYTE: AN EASY WAY TO WTHDRAW HEAVY METAL IONS]]></title>
			<description><![CDATA[CONTEXT
Industrial effluents usually comprises highly toxic dissolved metals (Ag, Au, Cd, Cu, Hg, Ni, Zn, Pd et Pb)
making them hazardous for the environment. Treating such effluents is a major issue especially that more and more regulatory measures are implementated to avoid any pollution of the environnement. Therefore numerous chemical processes exist to decontaminate these effluents but none of them are actually fully efficient and most of them use expensive reactants. If we add the fact that pollutant such as noble metals can have a high value afterward, there definitely exists a demand for a fully efficient inexpensive process.In the present work, the inventors have developped a decontamination process of an effluent comprising dissolved metallic cations.
This decontamination is allowed through the use of polyelectrolytes in order to form colloids with said cations. The obtained colloids then sediment. This is a one-step process to trap said cations.&nbsp;
DEVELOPMENT STAGE

-&nbsp;the decontamination process has been validated in an industrial pilot configuration.
BENEFITS
The present process aims at offering an efficient, inexpensive, one-step, fast decontamination treatment without use of toxic side products. Another benefit is that used reactants and extracted pollutant agents can be reused thereafter.
This invention not only decontaminate but also recycle!
As already stated, such process aims at decontaminating used industrial effluents containing cations.


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			<pubDate><![CDATA[Fri, 06 Apr 2012 00:18:22 +0200]]></pubDate>
			<link>http://www.technologie.oseo.fr/repertoires_d_offres/10/10_2/10_2_1/03981_01?lang=ang</link>
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			<title><![CDATA[HandTouchTissue device]]></title>
			<description><![CDATA[The new easy to use device measures simultaneously softness (vibratory measurement) and stiffness (force sensors) of fabric in a liable, objective and reproducible way. Any manufacturing drift can be detected on time thanks to successive or continuous testing of fabric samples from production. A single quality control reference is created and applicable in different geographical production sites at the national or international level. Fabric manufacturing quality is kept constant.
- Textiles manufacturing industry (woven and nonwoven materials)
- Luxury clothing industry
Vibratory signatures of cashmere, silk, jersey and paper handkerchiefs were obtained and characterized.
- objective, liable and reproducible characterization of fabric quality, compared to human testing
- creation of a single control quality reference
- useful device to maintain constant manufacturing quality worldwide
- easy to use device 
- woven and nonwoven fabric testing
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			<pubDate><![CDATA[Fri, 30 Mar 2012 11:33:14 +0200]]></pubDate>
			<link>http://www.technologie.oseo.fr/repertoires_d_offres/3/3_5/lst25mat?lang=ang</link>
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